Effect of AcSDKP on the proliferation and collagen synthesis in cultured rat cardiac fibroblasts stimulated by PDGF.
- Author:
Xi-Ling ZHU
1
;
Li-Ping WANG
;
Fang YANG
;
Rui-Min WANG
;
Wen-Dong MA
;
Ling LUO
;
Wan-Mi HU
;
Li-Juan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; Cells, Cultured; Collagen; biosynthesis; Fibroblasts; cytology; drug effects; metabolism; Myoblasts, Cardiac; cytology; drug effects; metabolism; Oligopeptides; pharmacology; Platelet-Derived Growth Factor; pharmacology; Rats; Rats, Wistar
- From: Chinese Journal of Applied Physiology 2007;23(1):66-69
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate whether AcSDKP can inhibit proliferation and collagen synthesis in cultured rat cardiac fibroblasts mediated by PDGF.
METHODSNeonatal rat cardiac fibroblasts were isolated. The cell proliferation was observed by 3H-proline incorporation assay.
RESULTSOn the culture of 0.4% FBS, PDGF stimulated cardiac fibroblasts proliferation and collagen synthesis with a dose-dependent manner at the concentrations from 1 ng/ml to 20 ng/ml, in which 10 ng/ml PDGF reached its peak. AcSDKP at the concentration from 10(-10) mol/L to 10(-8) mol/L could inhibit cardiac fibroblasts proliferation and collagen synthesis mediated by PDGF. 10(-9) mol/L AcSDKP attained its peak on inhibiting cardiac fibroblasts proliferation and collagen synthesis.
CONCLUSIONAcSDKP can inhibit proliferation and collagen synthesis in cultured rat cardiac fibroblasts mediated by PDGF.
