Effects of IL-1beta on inducible nitric oxide synthase-nitric oxide system activity in arginine vasopressin-induced rat cardiac fibroblasts.
- Author:
Yan-Hong FAN
1
;
Lian-Yiu ZHAO
;
Hai-Changm WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arginine Vasopressin; pharmacology; Fibroblasts; drug effects; metabolism; Interleukin-1beta; pharmacology; Male; Myocytes, Cardiac; drug effects; metabolism; Nitric Oxide; metabolism; Nitric Oxide Synthase Type II; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2007;23(1):70-73
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo explore the effects of interleukin-1beta (IL-1beta) on inducible nitric oxide synthase (iNOS)-nitric oxide (NO) system activity in arginine vasopressin (AVP)-induced rat cardiac fibroblasts (CFs).
METHODSCFs were isolated by trypsin digestion method. Nitric acid reductase method, spectrophotometry and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect NO contents, NOS activity and iNOS mRNA expression.
RESULTSAVP significantly increased iNOS mRNA expressions, NOS activity and NO contents (P < 0.05) in CFs. IL-1beta enhanced the effects of AVP on iNOS-NO system activity in a concentration-dependent manner, moreover the iNOS mRNA expressions, NOS activity and NO contents of AVP + 3 ng/ml, AVP + 5 ng/ml IL-1beta group were both significantly higher than those of AVP group (P < 0.05). But when IL-1beta concentration increased to 5 ng/ml, the iNOS mRNA expressions, NOS activity and NO contents did not increase accordingly, slightly decreased instead.
CONCLUSIONWithin certain range of concentrations IL-1beta cooperates with AVP to increase iNOS-NO system activity in CFs.
