Human inward rectifying potassium current and Kir2.1 mRNA expression in myocytes isolated from patients with chronic atrial fibrillation.
- Author:
Yu ZHANG
1
;
Xiao-rong ZENG
;
Yan YANG
;
Biao ZHANG
;
Zhi-fei LIU
;
Miao-ling LI
;
Wen ZHOU
;
Jie PEI
Author Information
- Publication Type:Journal Article
- MeSH: Atrial Fibrillation; genetics; metabolism; physiopathology; Gene Expression; Humans; Myocytes, Cardiac; metabolism; physiology; Patch-Clamp Techniques; Potassium Channels, Inwardly Rectifying; genetics; metabolism; RNA, Messenger; genetics
- From: Chinese Journal of Cardiology 2006;34(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the changes of both inward rectifying K(+) (Kir) current(I(k1)) density and mRNA expression level of Kir2.1, a major subfamily of Kir in chronic human atrial fibrillation (CAF) with those in normal sinus rhythm (NSR).
METHODSI(k1) density was measured with whole-cell patch clamp technique in single myocyte isolated by an enzymatic dissociation method from right atrial appendages in patients with CAF (n = 8) and those with NSR (n = 12). The mRNA expression levels of Kir2.1 was determined in right atrial appendages from CAF (n = 19) and NSR (n = 18) by semiquantitative reverse-transcription polymerase chain reaction (RT-PCR).
RESULTThe average resting membrane potentials were similar between CAF and NSR (-78.95 mV +/- 4.67 mV and -70.22 mV +/- 11.08 mV, P>0.05). I(k1) density in single myocyte significantly increased at hyperpolarized potential level (-100 mV) in CAF compared to that in NSR (-9.59 pA/pF +/- 2.47 pA/pF vs. -5.58 pA/pF +/- 2.52 pA/pF, P<0.01). The mRNA level of Kir2.1 was also significantly higher in CAF than that of NSR (0.50+/-0.16 vs. 0.34+/-0.09, P<0.05).
CONCLUSIONThe data suggest that Kir2.1 up-regulation and I(k1) current increase might contribute to the electrical remodeling in CAF patients.
