Fast determination of phenolic acids in Lycium ruthenicum murr juice by solid phase extraction and HPLC.
- Author:
Chen CHEN
1
;
Huaixiu WEN
;
Xiaohui ZHAO
;
Yanduo TAO
;
Yun SHAO
;
Lijuan MEI
Author Information
- Publication Type:Journal Article
- MeSH: Beverages; analysis; Chromatography, High Pressure Liquid; methods; Hydroxybenzoates; analysis; isolation & purification; Lycium; chemistry; Reproducibility of Results; Solid Phase Extraction; methods; Time Factors
- From: China Journal of Chinese Materia Medica 2011;36(7):896-898
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative method for determination of gallic acid, original catechins, catechins, chlorogenic acid, caffeic acid, syringic acid in Lycium ruthenicum.
METHODThe sample was separated on an ODS column (4. 6 mm x 250 mm, 5 pnm), eluted with methanol and water containing 0. 1% acid with detected wavelength at 280 nm, and flow rate at 1. 0 mL x min(-1).
RESULTThe linearity of six components were good (r = 0.9999). The average recoveries (n=5) of the six constituents were 97. 70% (RSD 2.3%), 99.64% (RSD 1.8%), 100.7% (RSD 2.1%), 99.98% (RSD 2.6%), 99.60% (RSD 2.2%), 99.04% (RSD 2.4%), respectively.
CONCLUSIONThe method is rapid and precise. It can be used for quality control of Lycium ruthenicum juice.
