Simultaneous determination of acteoside, oleanolic acid and ursolic acid in flower of Campsis grandiflora by HPLC.
- Author:
Qiao ZHANG
1
;
Juan SHEN
;
Yujie LIU
;
Yu'an BI
;
Zhenzhong WANG
;
Wei XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Bignoniaceae; chemistry; Chromatography, High Pressure Liquid; methods; Flowers; chemistry; Glucosides; analysis; Oleanolic Acid; analysis; Phenols; analysis; Spectrophotometry, Ultraviolet; methods; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2011;36(8):1043-1045
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for the determination of acteoside, oleanolic acid and ursolic acid in flowers of Campsis grandiflora.
METHODThe analysis was carried out on an Agilent ZORBAX Eclipse XDB-18 column eluted with methanol and 0.1% phosphoric acid in gradient mode. The detection wavelength was set at 334 mn at 0-30 min and 210 nm at 30-60 min.
RESULTThe peak areas and concentrations have a good linear relationship at 0.025 9-0.258 g x L(-1) for acteoside, 0.100-1.00 g x L(-1) for oleanolic acid and 0.104-1.04 g x L(-1) for ursolic acid, respectively. The average recoveries were 98.9%, 99.3% and 99.40%, respectively.
CONCLUSIONThe method can determinate the concentration of acteoside, oleanolic acid and ursolic acid simultaneously. It can be used for the quality control of flower of C. grandiflora.
