HPLC determination of four components in Tibetan medicine Dangzuo of different Tibetan regions.
- Author:
Cen LI
1
;
Yuancan XIAO
;
Hongxia YANG
;
Yuzhi DU
;
Lixin WEI
Author Information
- Publication Type:Journal Article
- MeSH: Acrolein; analogs & derivatives; analysis; isolation & purification; Alkaloids; analysis; isolation & purification; Benzodioxoles; analysis; isolation & purification; Chalcone; analogs & derivatives; analysis; isolation & purification; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Gallic Acid; analysis; isolation & purification; Medicine, Tibetan Traditional; Piperidines; analysis; isolation & purification; Plant Components, Aerial; chemistry; Plant Extracts; analysis; isolation & purification; Polyunsaturated Alkamides; analysis; isolation & purification; Quality Control; Quinones; analysis; isolation & purification; Reference Standards; Spectrophotometry, Ultraviolet; methods
- From: China Journal of Chinese Materia Medica 2011;36(8):1046-1049
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Tibetan medicine Dangzuo, and to compare the content of four active components in Dangzuo of different Tibetan regions.
METHODThe separation was carried out on a Waters XTerra RP-C18 column ( 4.6 mm x 250 mm, 5 microm). The mobile phases were methanol and water, all contained 0.1% glacial acetic acid, for gradient elution. The gradient program was as follows: 0-22.5 min, methanol was changed from 5% to 50%; 22.5-40 min, changed to 80% 80:20. The flow rate was 1.0 mL x min(-1). The detection wavelength was 270 nm. The reference wavelength was 500 nm.
RESULTThe linear ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine were 0.040-0.640 microg (r = 0.999 8), 0.090-1.440 microg (r = 0.999 9), 0.031-0.500 microg (r = 0.999 9 ) and 0.092-41.477 microg (r = 0.998 9), respectively. The average recoveries (n = 6) were 97.42% (RSD 1.9%), 97.55% (RSD 2.9%), 98.69% (RSD 0.96%) and 96.72% (RSD 4.0%), respectively. The content ranges of gallic acid, hydroxysafflor yellow A, cinnamic aldehyde and piperine in Dangzuo samples of different Tibetan regions were 0.11341.69 mg x g(-1), 0.889-1.51 mg x g(-1), 0.000-40.606 mg x g(-1) and 1.96-2.73 mg x g(-1), respectively.
CONCLUSIONThe method is a simple and effective for quality control of Tibetan medicine Dangzuo.
