Protective effects and mechanism of Inonotus obliquus on asthmatic mice.
- Author:
Guanghai YAN
1
;
Guangyu JIN
;
Liangchang LI
;
Xiangzheng QIN
;
Changji ZHENG
;
Guangzhao LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Asthmatic Agents; isolation & purification; pharmacology; Asthma; drug therapy; metabolism; pathology; Basidiomycota; chemistry; Basophils; drug effects; metabolism; Bronchoalveolar Lavage Fluid; cytology; immunology; Disease Models, Animal; Interferon-gamma; drug effects; metabolism; Interleukin-13; metabolism; Interleukin-4; metabolism; Interleukin-5; metabolism; Lung; pathology; Lymphocytes; drug effects; metabolism; Mice; Mice, Inbred BALB C; Neutrophils; drug effects; metabolism; Phytotherapy; Plant Extracts; pharmacology; p38 Mitogen-Activated Protein Kinases; drug effects; metabolism
- From: China Journal of Chinese Materia Medica 2011;36(8):1067-1070
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the protective effects and mechanism of ethanol extract of Inonotus obliquus (EEIO) injection on asthmatic mice.
METHODOVA was injected intraperitoneally and inhaled to produce the asthmatic model. Thirty two mice were randomly divided into four groups: control group, asthma group and I. obliquus groups of high and low dose. The concentrations of IL-4, IL-5, IL-13 and IFN-gamma in BALF, the phosphor-p38 MAPK in lung tissues were respectively measured by ELISA and Western blotting. The number of inflammatory cells in BALF and histopathology changes were observed.
RESULTIn asthmatic group, the number of inflammatory cells and the concentrations of IL-4, IL-5, IL-13 in BALF and phospho-p38 MAPK in lung tissue were higher, while IFN-gamma were lower than those in normal control mice (P < 0.05). In I. obliquus group, the number of inflammatory cells, the concentrations of IL-4, IL-5, IL-13 in BALF and phosphor-p38 MAPK in lung tissue were lower, but were higher than those in normal control mice (P < 0.05), and histropathology damage was alleviated significantly. There was no significant difference observed among the efficacies in the I. obliquus groups of high and low dose.
CONCLUSIONp38 MAPK may play a role in pathological process of asthma. I. obliquus effectively treats asthma by inhibiting the expression of phosphor-p38 MAPK, correcting the unbalance of IFN-gamma/IL-4 and decreasing the number of inflammatory cells.
