Study on transportation of Xiaochaihu Tang in Caco-2 cell model.
- Author:
Liangchun YAN
1
;
Qingchun LIU
;
Junning ZHAO
;
Yueqi BI
;
Jinhai YI
Author Information
- Publication Type:Journal Article
- MeSH: Biological Transport, Active; Caco-2 Cells; metabolism; Cell Line, Tumor; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; pharmacokinetics; Flavanones; analysis; metabolism; Flavonoids; analysis; metabolism; Glucosides; analysis; metabolism; Glycyrrhizic Acid; analysis; metabolism; Humans
- From: China Journal of Chinese Materia Medica 2011;36(8):1087-1090
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the transportation of Xiaochaihu Tang in Caco-2 cell model.
METHODThe safety concentration of Xiaochaihu Tang in Caco-2 cells was determined by MTT assay. Then the Caco-2 cell model was used to investigate the bi-directional transportation of Xiaochaihu Tang. The multicomponents of Xiaochaihu Tang and the influence of time were measured by high performance liquid chromatography (HPLC).
RESULTThe P(app) values of wogonoside and wogonin were (1.23 +/- 0.09) x 10(-6), (1.07 +/- 0.89) x 10(-5) cm x s(-1) from the AP side to BL side, and (2.12 +/- 0.19) x 10(-6) and (7.12 +/- 1.02) x 10(-6) cm x s(-1) from the BL side to AP side, respectively. The P(appAP --> BL)/P(app BL --> AP) ratio of wogonoside and wogonin were 0.58 and 1.49, respectively. Baicalin, baicalein and glycyrrhizic acid could not permeate the Caco-2 cell model.
CONCLUSIONThe transportation of wogonoside and wogonin in Caco-2 cell model may be a passive transportation.
