Determination of synephrine and arecoline contents in Xiao'er Xiaoji Zhike oral liquid.
- Author:
Sihe KANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Arecoline; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Synephrine; analysis
- From: China Journal of Chinese Materia Medica 2011;36(10):1298-1300
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a HPLC method for the determination of synephrine and arecoline contents in Xiao'er Xiaoji Zhike oral liquid.
METHODThe analysis was performed on a Symmetry C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetonitrile-methanol-0.1% acetic acid solution (containing potassium dihydrogen phosphate 0.6 g and SDS 1.0 g per 1 000 mL) (15: 30: 55) as mobile phase. The detection wavelength was set at 215 nm.
RESULTThe synephrine and arecoline concentrations had good linear relationship between 0.281 5-2.815 and 0.040 16-0.401 6 microg (r > 0.999 7), and the average recoveries of the two compounds were 97.1% (RSD 1.4%) and 100% (RSD 1.3%), respectively.
CONCLUSIONThe method is simple, accurate and sensitive.