HPLC-TOF-MS analysis of metabolites of Diffusa effective extracts in rats.
- Author:
Lizhi YAN
1
;
Yingfeng WANG
;
Qirong YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; administration & dosage; analysis; metabolism; Hedyotis; chemistry; Male; Mass Spectrometry; methods; Rats; Rats, Sprague-Dawley
- From: China Journal of Chinese Materia Medica 2011;36(10):1301-1304
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze p-coumaril acid, trans-6-O-p-coumaroyl scandoside methyl ester and its metabolites in rat plasma after intragastric administration of exocarpium Diffusa effective extracts.
METHODRat blood samples were collected 1.0 h after oral administration of 0.4 g x kg(-1) exocarpium Diffusa effective extracts, and then were analyzed by using HPLC-Q-TOF-MS.
RESULTIn rat plasma, only one metabolite was detected. The structure was identified by reference substance to be p-coumaril acid.
CONCLUSIONThe methyl glucoside metabolite in rat from the main component parts of trans-6-O-p-coumaroyl scandoside methyl ester droppings of vines in Diffusa effective extracts is p-coumaril acid. This experiment provides a theortical basis for studying chemical compositions and pharmacodynamic action of Diffusa.
