Simultaneous determination of 4 major components in semen cassiae obtusifoline by HPLC.
- Author:
Huijuan SU
1
;
Zhuju WANG
;
Liying TANG
Author Information
- Publication Type:Journal Article
- MeSH: Cassia; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Seeds; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(10):1327-1329
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC method for the simultaneous determination of 4 major components in semen cassiae obtusifoline and provide valuable data for quality control of semen cassiae obtusifoline.
METHODKromasil 100-5 C18 column (4.6 mm x 250 mm, 5 microm). Eluted with water: acetonitrile: tetramethylene oxide: glacial acetic acid (100: 23: 5:1). Detecte at 278 nm. The flow rate was 1.0 mL x min(-1).
RESULTFour compositions were separated well. Good linearies were obtained within the range of 0.274-1.37 microg, 0.153-0.765 microg, 0.302-1.51 microg and 0.052-0.26 microg for rubrofusarin gentiobioside, casside, aurantio-obtusin-6-O-beta-D- glucopyranoside, casside B respectively. The average recoveries were 100.9% (RSD 1.8%),101.2% (RSD 1.2%), 99.40% (RSD 2.2%), 100.5% (RSD 1.6%).
CONCLUSIONThe method is accurate, reliable and convenient. It can be used to control the quality of semen cassiae obtusifoline and its products.
