Effects of mangiferin on cytokines in rats with chronic bronchitis and expression of macrophage COX-2 in mice.
- Author:
Qin WANG
1
;
Jiagang DENG
;
Ke YANG
;
Lancheng XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bronchitis, Chronic; drug therapy; genetics; immunology; Cell Line; Cyclooxygenase 2; genetics; immunology; Cytokines; genetics; immunology; Drugs, Chinese Herbal; administration & dosage; pharmacology; Gene Expression; drug effects; Humans; Macrophages; drug effects; enzymology; immunology; Male; Mice; Rats; Rats, Sprague-Dawley; Xanthones; administration & dosage; pharmacology
- From: China Journal of Chinese Materia Medica 2011;36(10):1348-1352
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of anti-inflammatory effect of mangiferin.
METHODThe model of chronic bronchitis in rat was established by LPS + smoke. The activity of SOD, content of MDA and NO in BALF and serum, content of TNF-alpha and IL-8 were determined. The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS in mice was detected by RT-PCR.
RESULTThe activity of SOD, the content of NO in BALF and serum in rat with chronic bronchitis were significantly higher with high, medium and low-dose of lg mangiferin (400,200,100 mg x kg(-1)), while the content of MDA, and the content of TNF-alpha and IL-8 in lung tissues were lower. The expression of RAW264.7 macrophage COX-2 mRNA induced by LPS was significantly reduced by mangiferin with 200,100, 50 micromol x L(-1).
CONCLUSIONThe anti-inflammatory mechanism of mangiferin is to relieve inflammation by raising the activity of SOD and content of NO and reducing the content of MDA and the expression of TNF-alpha, IL-8 and COX-2 mRNA.
