Comparasion of polysaccharides in parent root, daughter root and rootlet of Aconitum carmichaeli.
- Author:
Yonglei LU
1
;
Haibo BU
;
Lei YANG
;
Xiangri LI
;
Fei LI
Author Information
- Publication Type:Journal Article
- MeSH: Aconitum; metabolism; Magnetic Resonance Spectroscopy; Plant Roots; metabolism; Polysaccharides; metabolism; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2011;36(9):1154-1157
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the polysaccharides contents and monosaccharide compositions in parent root, daughter root and rootlet of Aconitum carmichaeli.
METHODThe conversion coefficient of A. carmichaeli polysaccharide to glucose was obtained by refined polysaccharides, and then the contents of crude polysaccharides in parent root, daughter root and rootlet were determined by sulfuric-phenol spectrometry method; analysis of monosaccharide compositions in polysaccharides from A. carmichaeli was carried out by pre-column derivatization high performance liquid chromatography with 1-phenyl-3-methyl-5-pyrazolone (PMP).
RESULTThe contents of polysaccharides in parent root, daughter root and rootlet were 22.02%, 33.53% and 6.10%, respectively. Parent root, daughter root and rootlet mainly contained glucose, and in addition they contained a small amount of galacturonic acid, galactose and arabinose. Daughter root contained mannose yet, and rootlet still contained mannose, rhamnose and xylose.
CONCLUSIONThe method is simple, rapid, and accurate. The content of polysaccharide in rootlet is lowest, and monosaccharide compositions in rootlet are significantly different from parent root and daughter root.
