Expressions of bacterial 16S rRNA, IL-1beta, TNF-alpha and NGF in prostate tissues.
- Author:
Hui XIE
1
;
Hui-Cong HUANG
;
Yi-Rong YANG
;
Qiu-Xiang HE
;
Qi-Jian ZHU
;
Jian-Ou CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Chronic Disease; Genes, rRNA; Humans; Interleukin-1beta; metabolism; Male; Nerve Growth Factor; metabolism; Prostate; metabolism; microbiology; pathology; Prostatitis; metabolism; microbiology; pathology; RNA, Bacterial; genetics; RNA, Ribosomal; RNA, Ribosomal, 16S; genetics; Tumor Necrosis Factor-alpha; metabolism; Young Adult
- From: National Journal of Andrology 2010;16(1):40-43
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of bacteria in the etiology of chronic prostatitis.
METHODSA total of 162 complete prostate specimens were obtained at autopsy from organ donors (aged 20 -38 yr) who died of non-prostatic diseases. Each of the samples from the peripheral zone of the prostate was divided into two parts, one for routine pathological examination and immunohistochemical studies of interleukin (IL)-1beta, tumor necrosis factor-alpha (TNF-alpha) and the nerve growth factor (NGF), and the other for PCR assay to detect the bacterial 16S rRNA gene (16S rDNA).
RESULTSFifty-one (31.5%) of the total specimens presented pathological changes of chronic prostatitis, of which 44 had mild focal stromal, 5 mild focal stromal and periglandular and 2 mild focal periglandular inflammation. The positive rate of 16S rDNA was 19.1% (31/162), 51.0% (26/51) in the chronic prostatitis and 4.5% (5/111) in the non-prostatitis specimens (chi2 = 29.783, P < 0.01). In the specimens with chronic prostatitis, the expressions of IL-1beta, TNF-alpha and NGF were significantly higher in the 16S rDNA positive than in the 16S rDNA negative group (P < 0.01).
CONCLUSIONBacterial inflammation may play an important role in the etiology of chronic prostatitis.
