M. tuberculosis Somatic Antigen Specific CD8+T cell Responses in BCG-Vaccinated Subjects.
10.4046/trd.2005.59.3.272
- Author:
Jang Eun CHO
1
;
Sang Nae CHO
;
Kyung Wha LEE
;
Seung Kyu PARK
;
Sungae CHO
Author Information
1. Department of Microbiology, Brain Korea 21 project for Medical Sciences, Yonsei University Medical Center, Korea. scho@pasteur.or.kr
- Publication Type:Original Article
- Keywords:
CD8+T cells;
cytotoxicity;
M. tuberculosis somatic antigens
- MeSH:
Enzyme-Linked Immunospot Assay;
Mycobacterium bovis;
Tuberculosis*
- From:Tuberculosis and Respiratory Diseases
2005;59(3):272-278
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The immune responses mediated by CD8+T cells are known to be significant in controlling M. tuberculosis infections. In order to determine the role of cytotoxic CD8+T cells in the protective immune mechanism in latently infected subjects, this study examined whether or not the cytotoxic immune responses of CD8+T cells specific to the M. tuberculosis somatic antigens are induced in BCG vaccinated healthy subjects. METHODS: Cytotoxicity and IFN-gamma elispot assays were used to investigate the activities of CD8+T cells specific for the thyA30-38 peptide epitope in circulating peripheral blood mononuclear cells (PBMC) from BCG-vaccinated HLA-A*0201 and A*0206 subjects. RESULTS: The results indicate the cytotoxic and IFN-gamma immune responses of CD8+T cells specific for thyA30-38 were induced in BCG vaccinated healthy subjects. CONCLUSION: The cytotoxic and IFN-gamma responses by CD8+T cells specific for the M. tuberculosis somatic antigens are induced in BCG-vaccinated subjects, and appear to be involved in the protective immune mechanism in latently infected people against a M. tuberculosis infection.
