Determination of sperm DNA integrity by improved sperm chromatin structure assay test.
- Author:
Jing CAI
1
;
Pei-Yan LIANG
;
Biao YIN
;
Jin-Ming LIANG
;
Qi LIN
;
Yong ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Chromatin; ultrastructure; DNA; analysis; Genetic Techniques; Humans; Male; Reference Values; Semen Analysis; Sperm Motility; genetics; Spermatozoa; chemistry
- From: National Journal of Andrology 2009;15(12):1116-1119
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search for a simple, quick and accurate method for the sperm DNA integrity test by modifying the conventional sperm chromatin dispersion (SCD) test, and to investigate the reference value range of normal sperm DNA integrity by the improved SCD assay.
METHODSWe modified the conventional SCD test in its procedures, reagents preparing and utensils to be used, compared the improved method with the conventional one, and detected 293 normal semen samples for sperm DNA integrity by the modified SCD test.
RESULTSAn improved SCD test was established, and no statistical differences were found between the results of the modified and conventional methods. The reference value range was determined by the improved SCD assay for normal sperm DNA integrity, which was < 32.58% for abnormal sperm DNA integrity.
CONCLUSIONThe improved SCD test could detect sperm DNA integrity more quickly, and it reduced the cost in reagents.
