Expressions of transforming growth factor-beta(1) and Smad4 in rat models of chronic nonbacterial prostatitis and their clinical significance.
- Author:
Yang-Min WANG
1
;
Shou-Lin LU
;
Zhi-Qiang ZHAO
;
Yu-Hai ZHAO
;
Nan WANG
;
De-Shan JING
;
Yong-Chao DONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Male; Prostate; metabolism; Prostatitis; metabolism; pathology; Rats; Rats, Sprague-Dawley; Smad4 Protein; metabolism; Transforming Growth Factor beta1; metabolism
- From: National Journal of Andrology 2010;16(6):490-494
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expressions of transforming growth factor-beta(1) and Smad4 in the prostatic tissue of rat models of chronic nonbacterial prostatitis (CNP), and to explore the mechanisms of CNP and its fibrosis.
METHODSSixty 6-month-old SD rats were randomly allocated into three groups of equal number: normal control, 30 d CNP model and 45 d CNP model, the models made by castration + high-dose intramuscular injection of estradiol benzoate. The expressions of TGF-beta1 and Smad4 in the prostatic tissue were detected by immunohistochemistry and Western blot.
RESULTSCompared with the normal controls, the 30 d and 45 d CNP rat models showed a significantly increased expression of TGF-beta1 and decreased expression of Smad4 (P < 0.05), even more significantly in the 45 d than in the 30 d group. And the expression of TGF-beta1 was negatively correlated with that of Smad4 in the CNP rat models.
CONCLUSIONTGF-beta1 and Smad4 may be involved in the pathogenesis of CNP, and prostatic fibrosis may make the condition difficult to cure.
