Effect of progesterone on the expression of GLUT in the brain following hypoxic-ischemia in newborn rats.
- Author:
Dong-Liang LI
1
;
Hua HAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Glucose Transporter Type 1; genetics; metabolism; Glucose Transporter Type 3; genetics; metabolism; Hippocampus; metabolism; Hypoxia-Ischemia, Brain; metabolism; Progesterone; physiology; Random Allocation; Rats; Rats, Sprague-Dawley; Up-Regulation; physiology
- From: Chinese Journal of Applied Physiology 2008;24(3):353-355
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the expression of GLUT1 and GLUT3 in the hippocampus after cerebral hypoxic-ischemia (HI) in newborn rats and the effect of progesterone (PROG) on them.
METHODSForty newborn SD rats were randomly divided into four groups: normal group, sham-operated group, hypoxic-ischemic group and progesterone group. Model of hypoxic-ischemia encephalopathy (HIE) was established in the 7-day-old newborn SD rats. Immunohistochemical method was applied to detect the expression of GLUT1 and GLUT3 in hippocampus.
RESULTSGLUT1 and GLUT3 were slightly seen in normal and sham operation group, there was no obviously difference between the two groups (P > 0.05). The expression of GLUT1 and GLUT3 in hypoxic-ischemia group were all higher than that in sham operated group (P < 0.05). Not only the expression of GLUT in progesterone group were significantly higher than that in sham operated group (P < 0.01), but also than that in hypoxic-ischemia group (P < 0.05).
CONCLUSIONPROG could increase the tolerance of neuron to hypoxic-ischemia with maintaining the energy supply in the brain by up-regulating GLUT expression.
