Ion channel mechanism of regulatory volume decrease in human epithelial cells.
- Author:
Lu Ping SHI
1
;
Yi Min ZANG
;
Xiao Li HOU
;
Jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Cell Size; drug effects; Chloride Channels; antagonists & inhibitors; metabolism; Epithelial Cells; cytology; Humans; Hypotonic Solutions; Intestine, Small; cytology; Potassium Channel Blockers; pharmacology; Potassium Channels; metabolism; Potassium Channels, Calcium-Activated; metabolism
- From: Chinese Journal of Applied Physiology 2008;24(3):356-360
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the regulatory volume decrease (RVD) process of human intestine cells and investigate its ion channel mechanism.
METHODSCultured human intestine cells were exposed to hypotonic solution and the cell volume was measured using Coulter Counter System. RT-PCR was explored to detect the mRNA expression of Ca(2+) -activated K+ channel.
RESULTSHuman intestine cells showed a RVD process and this process could be blocked by Cl- channel blocker NPPB and K+ channel blocker TEA. Further results demonstrated the subtype of K+ channel involved in RVD was an intermediate-conductance, Ca(2+) -activated K+ channel (IK) because of its high sensitivity to clotrimazole. RT-PCR results also showed the expression of IK in this cell line.
CONCLUSIONThe RVD process of intestine cell was dependent on the parallel activation of Cl- channel and K+ channel. The subtype of K+ channel in volved in the RVD process was IK channel.
