Comparisons of several methods used to isolate outer hair cells and morphological observations in cochlea.
- Author:
Yuan TIAN
1
;
Li YU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Separation; methods; Cochlea; cytology; physiology; Female; Guinea Pigs; Hair Cells, Auditory, Outer; cytology; physiology; Male; Silver Staining
- From: Chinese Journal of Applied Physiology 2008;24(3):373-376
- CountryChina
- Language:Chinese
-
Abstract:
AIMThe comparison of several methods which were used to isolate cochlear outer hair cells and the observations of morphology were researched.
METHODSThree different separating methods of outer hair cells in cochlea were adopted; the morphology of outer hair cells in cochlea and the morphology of cochlear stretched preparation in Silver Nitrate staining were also investigated.
RESULTSSingle alive OHC in cochlea was disassociated by all methods, with microscope and cochlear stretched preparation's staining. We could also observe appearances and distributions of OHC in cochlea.
CONCLUSIONIt is successful to isolate single alive cochlear OHC, and it will be very important to investigate deeply normal physiological functions and changes of functions and morphology in some pathologic status in cochlear OHCs.
