Expression of vascular smooth muscle cell markers during early stage of embryonic stem cell-derived embryoid bodies differentiation.
- Author:
Ya-ling HAN
1
;
Yan-ping XIAO
;
Yan-mei QI
;
Jian KANG
;
Cheng-hui YAN
Author Information
- Publication Type:Journal Article
- MeSH: Actins; genetics; metabolism; Animals; Biomarkers; metabolism; Cell Differentiation; Cells, Cultured; Embryonic Stem Cells; cytology; metabolism; Mice; Microfilament Proteins; genetics; metabolism; Muscle Proteins; genetics; metabolism; Muscle, Smooth, Vascular; cytology; Nuclear Proteins; genetics; metabolism; Proto-Oncogene Proteins c-sis; metabolism; RNA, Messenger; genetics; metabolism; Trans-Activators; genetics; metabolism
- From: Chinese Journal of Applied Physiology 2008;24(4):385-390
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe expression regularity of SMalpha-actin, SM22alpha, myocardin and SMMHC during early embryonic vascular development, and to initially investigate the differentiation effect of platelet derived growth factor-BB (PDGF-BB) on vascular smooth muscle cells (VSMCs) during that period.
METHODSMurine embryonic stem cell line expressing the enhanced green fluorescent protein (GFP) under the transcriptional control of the smooth-muscle-specific SM22alpha promoter was used to make embryoid bodies,and to analyze the expression regularity of SMalpha-actin, SM22alpha, myocardin and SMMHC by immunofluorescence stainings, RT-PCR and Western blot. Then AG1296 (PDGF receptor inhibitor) 0 micro-mol/L(control group), 10 micromol/L and 50 micromol/L were used to treat EBs respectively in order to analyze the differences of SMa-actin, SM22alpha, myocardin and SMMHC at gene and protein levels among the three groups.
RESULTSSMalpha-actin, myocardin, SM22alpha and SMMHC expression in EBs were found to begin at day 0 (ESCs), 8, 11, 13 respectively during early embryonic vascular development. There were no clear differences in SMa-actin, SM22alpha, myocardin and SMMHC protein expression and SM22alpha, myocardin and SMMHC mRNA level among the three groups of different concentrations of AG1296.
CONCLUSIONA spontaneous VSMCs differentiation occurs during EBs development, SMalpha-actin is the first to be detected,the following are myocardin, SM22a and SMMHC. PDGF-BB may not be indispensable for the regulation of expression of VSMCs markers during early EBs differentiation.
