Effect of ischemic preconditioning on lung injury induced by ischemia/reperfusion in the hind limbs of rats.
- Author:
Hong-bo GAO
1
;
Lin-jing ZHAO
;
Shu-yun DONG
;
Yan LIU
;
Bo ZHANG
;
Na ZHANG
;
Yin-huan WANG
;
Lian-yuan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; prevention & control; Animals; Extremities; blood supply; Ischemia; physiopathology; Ischemic Preconditioning; methods; Lung; blood supply; Male; Random Allocation; Rats; Rats, Wistar; Reperfusion Injury; metabolism; prevention & control
- From: Chinese Journal of Applied Physiology 2008;24(4):439-442
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of ischemic preconditioning on lung injury following ischemia/reperfusion (I/R) in the hind limbs of rats.
METHODSWistar rats were randomly divided into four groups (n=8): control group,limbs ischemia/reperfusion (LI/R) group, ischemia preconditioning (IPC) group and L-NAME group. At the end of the experiment, blood/gas analysis and the contents of serum MDA, NO, ET and lung tissue MDA, NO, ET, MPO were measured. Meanwhile, lung index and W/D) of lung were measured.
RESULTSAfter the rats' hind limbs suffered ischemia/reperfusion, the level of PaO2 decreased and the values of W/D, LI, MPO of the lung issure and MDA, NO, ET of plasma and lung all increased significantly in the LI/R group; but the ratio of NO/ET decreased. Compared with LI/R group, the contents of NO and ratio of NO/ET increased but other parameters decreased in the IPC group. Compared with IPC group, the contents of NO and ratio of NO/ET decreased, but other parameters increased in the L-NAME group.
CONCLUSIONThe IPC can attenuate lung injury following IR in the hind limbs of rats, which may correlated with the increase of NO.
