Effects of melatonin on anti-oxidative function of lung injury induced by lipopolysaccharide in rats and ICAM-1 expression.
- Author:
Jun-yi ZHANG
1
;
Chun-hua DING
;
Xiao-hua SUO
;
Cai-feng DONG
;
Yi-ling LING
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; chemically induced; metabolism; physiopathology; Animals; Antioxidants; pharmacology; Intercellular Adhesion Molecule-1; genetics; metabolism; Lipopolysaccharides; Male; Melatonin; pharmacology; Peroxidase; metabolism; Protective Agents; pharmacology; Random Allocation; Rats; Rats, Sprague-Dawley; Superoxide Dismutase; metabolism
- From: Chinese Journal of Applied Physiology 2008;24(4):464-467
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the protective effect of melatonin (MT) on lung tissues during acute lung injury (ALI) in rats and its possible mechanism.
METHODSAll rats were randomly divided into four groups: control group, lipopolysaccharide (LPS) group, dexamethasone (DEX) and MT treatment group. Myeloperoxidase (MPO) activity, superoxide dismutase (SOD) activity and malonaldehyde (MDA) content of lung tissues were detected at 3, 6 and 12 h after intratracheal instillation in each group. In addition, the expression of intercellular adhesion molecule-1 (ICAM-1) were observed through immunohistochemistry staining in lung tissues.
RESULTSCompared with control group, SOD activity decreased significantly in LPS group (P < 0.01), but MPO activity,MDA content and the expression of ICAM-1 increased obviously (P < 0.01). The administration of MT and DEX mitigated above changes significantly (P < 0.05 or P < 0.01).
CONCLUSIONMT possessed protective effect on lung tissues during ALI through scavenging free radicals and inhibiting the expression of ICAM-1 probably.
