The assay of transcriptional activation effect of TR3 and deletion mutation in the yeast two hybrid system.
- Author:
Xin-xing WANG
1
;
Zhi-hua YANG
;
Xiao-hua LIU
;
Dong-sheng GUO
;
Ling-jia QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Nuclear Receptor Subfamily 4, Group A, Member 1; genetics; physiology; Sequence Deletion; Transcriptional Activation; genetics; Two-Hybrid System Techniques
- From: Chinese Journal of Applied Physiology 2008;24(4):504-507
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo assay the transcriptional activation effect of TR3 and it's deletion mutation in yeast two hybrid system.
METHODSThe total length of TR3 and TR3/delta1-690 gene was amplified by PCR method and cloned into pGBKT7 vector. Bait vector of pGBKT7-TR3 and pGBKT7-TR3/delta1-690 was transformed into AH109 competence yeast. Then self activation of the recombination vector was tested by assay the activity of beta-galactosidae.
RESULTSThe pGBKT7-TR3 and pGBKT7-TR3/AM 690 vector was successfully constructed. The filter paper containing beta-galactosidae didn't changed to blue showed that the reporter gene wasn't activationed.
CONCLUSIONTR3 and TR3/delta1-690 hadn't the activity of transcriptional activation.
