Cardioprotection of testosterone in rat hearts subjected to ischemia/reperfusion.
- Author:
Jue WANG
1
;
Bo YANG
;
Qin GAO
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cardiotonic Agents; pharmacology; Hydrogen Peroxide; KATP Channels; metabolism; Male; Mitochondrial Membrane Transport Proteins; drug effects; Myocardial Reperfusion Injury; metabolism; physiopathology; prevention & control; Orchiectomy; Oxidative Stress; drug effects; Rats; Rats, Sprague-Dawley; Testosterone; pharmacology; therapeutic use
- From: Chinese Journal of Applied Physiology 2009;25(1):31-35
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate the chronic cardioprotection of testosterone against ischemia/reperfusion injury and acute effect against H2O2-stress injury.
METHODSThe vas deferens were ligated bilaterally and the testes removed from male Sprague-Dawley rats, and testosterone propionate was supplemented every day. Eight weeks after gonadectomy, all the hearts were mounted on a Langendorff apparatus to assess the level of lactate dehydrogenase (LDH) in the coronary effluent and the infarct size. Isolated adult ventricular myocytes were obtained by enzymatic dissociation, in which H2O2-stress injury model was copied. The myocyte contraction was determined, and mitochondrial reactive oxygen species (ROS) production was measured by loading with fluorescent probe DCFH-DA.
RESULTSIn gonadectomy model, pretreatment with testosterone propionate significantly decreases the LDH release and the infarct size. In the isolated myocytes model, testosterone attenuated the decreases of +/- dL/dtmax and dL which produced by H2O2-stress, and prevented the production of ROS induced by H2O2-stress. Co-treatment with atractyloside or 5-HD attenuated the effect of testosterone.
CONCLUSIONThe findings show the chronic cardioprotection of testosterone against ischemia/reperfusion injury and acute effect against H2O2-stress injury via opening of mitoK(ATP) channel or/and the inhibiting mitochondrial permeability transition pore.