The effects of Sphing-1-phosphate(S1P) on the potassium channel of the ventricular myocytes.
- Author:
Ming ZHAO
1
;
Wen-Jie ZHANG
;
Chun-Yan ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Delayed Rectifier Potassium Channels; drug effects; Female; Guinea Pigs; Heart Ventricles; cytology; metabolism; Lysophospholipids; pharmacology; Male; Myocytes, Cardiac; metabolism; Patch-Clamp Techniques; Potassium Channels; drug effects; Potassium Channels, Inwardly Rectifying; drug effects; Sphingosine; analogs & derivatives; pharmacology
- From: Chinese Journal of Applied Physiology 2009;25(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of Sphingosine-1-phosphate on the delayed rectifier potassium current (IK) and the inward rectifier potassium current (IK1) of guinea pig isolated ventricular myocytes.
METHODSWhole cell patch clamp technique was applied to record the delayed rectifier potassium current and the delayed rectifier potassium current of guinea pig isolated ventricular myocytes.
RESULTS(1) IK of S1P (1.1 micromol/L) decreased from (1.2 +/- 0.26)nA to (0.95 +/- 0.23)nA. While IK of S1P (2.2 micromol/L) decreased from (1.43 +/- 0.31)nA to (1.02 +/- 0.28)nA. There was significant difference compared to control group (P < 0.01, n = 6). The IK peak value was decreased from (1.29 +/- 0.26) nA to (1.26 +/- 0.37)nA at the group of S1P (1.1 micromol/L) plus suramin (200 micromol/L) and showed no significant difference compared to control group (P > 0.05, n = 6). (2) IK1 of S1P (1.1 micromol/L) decreased from (-8.94 +/- 2.01)nA to (-8.81 = 1.55)nA. While IK of S1P (2.2 micromol/L) decreased from (-8.86 +/- 1.59)nA to (-8.55 +/- 1.39)nA. There was no significant difference compared to control group (P > 0.05, n = 6).
CONCLUSIONS1P inhibits delayed rectifier potassium current of ventricular myocyte in guinea pig remarkably, S1P shows no effects on delayed rectifier potassium current of ventricular myocyte in guinea pig.
