Effect of gap junction on the cardioprotection of ischemic postconditioning in rat heart.
- Author:
Hong-Jiao MAO
1
;
Bao-Ping CHEN
;
Tu-Nan YU
;
Zhi-Guo YE
;
Xiang-Gui YUAN
;
Qiang XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Gap Junctions; physiology; Heptanol; pharmacology; Ischemic Postconditioning; methods; Male; Myocardial Ischemia; physiopathology; Myocardial Reperfusion Injury; physiopathology; prevention & control; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2009;25(1):60-64
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine whether the cardioprotection of ischemic postconditioning and heptanol in ischemic heart against ischemia/reperfusion (I/R) is mediated by gap junction.
METHODSThe effect of ischemic postconditioning, heptanol at different doses (0.03, 0.06, 0.30, and 0.60 mg/kg) and AAP10 (10 mg/kg) on the intact rat heart during 30 min ischemia and 2 h of reperfusion was observed. Ischemic postconditioning was achieved by 3 cycles of 10 s reperfusion/10 s regional ischemia starting at the beginning of the reperfusion. The infarct size and the arrhythmia scores were measured. The effect of ischemic postconditioning, heptanol at different doses (0.05, 0.10, 0.50 and 1.00 mmol/L) and AAP10 (1 x 10(-7)mol/L) on the isolated heart during 30 min ischemia and 2 h of reperfusion was observed. Ischemic postconditioning was achieved by 6 cycles of 10 s reperfusion/10 s global ischemia starting at the beginning of the reperfusion. The arrhythmia scores and conduction velocity of ventricle muscle were measured.
RESULTSIn the intact rat heart model, ischemic postconditioning and heptanol reduced infarct size and arrhythmia scores. In the Langendorff perfused rat heart model, ischemic postconditioning and heptanol reduced arrhythmia scores and conduction velocity of ventricle muscle. Administration of AAP10, an opener of gap junction attenuated the cardioprotection of ischemic postconditioning and heptanol.
CONCLUSIONThe cardioprotection of ischemic postconditioning and heptanol may be related to the attenuation of gap junction communication on myocardiac ischemia/reperfusion injury.