The expression of matrix metalloproteinase-2,9 on atherosclerosis in experimental rats by treatment of 2,3,4',5-tetrahydroxystilbene -2-0-beta-D glucoside.
- Author:
Xiao-Le XU
1
;
Feng LI
;
Chun-Hua WANG
;
Yan SHEN
;
Wei ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Atherosclerosis; drug therapy; metabolism; C-Reactive Protein; metabolism; Glucosides; isolation & purification; therapeutic use; Interferon-alpha; blood; Interleukin-6; blood; Male; Matrix Metalloproteinase 2; genetics; metabolism; Matrix Metalloproteinase 9; genetics; metabolism; Polygonum; chemistry; RNA, Messenger; genetics; metabolism; Rats; Rats, Sprague-Dawley; Stilbenes; isolation & purification; therapeutic use
- From: Chinese Journal of Applied Physiology 2009;25(1):91-95
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe the changes of MMP-2, 9 level on atherosclerosis in experimental rats by treatment of 2,3,4',5-tetrahydroxystilbene-2-0-beta-D glucoside (TSG) and to investigate the mechanism of TSG in stabilizing plaque and anti-atherosclerosis.
METHODSThe atherosclerosis model of rat was made by feeding high grease food and injecting VitD3. Sixty male SD rats were randomly divided into six groups: control, Simvastatin, model and TSG 120 mg x kg(-1) x d(-1), TSG 60 mg x kg(-1) x d(-1) and TSG 30 mg x kg(-1) x d(-1). After 12 weeks, several aorta were randomly tested, model and TSG 120 mg x kg(-1) x d(-1), TSG 60 mg kg(-1) x d(-1) and TSG 30 mg x kg(-1) x d(-1). After 12 weeks, several aorta were randomly tested, model made was successful when we found plaque. And after six weeks treating, the mRNA expressions of MMP-2 and MMP-9 were measured by RT-PCR. The activities of MMP-2 and MMP-9 were measured by Western blot. The levels of CRP, IL-6 and TNF-alpha in serum were measured in biochemical method.
RESULTSData of the study demonstrated that the level of TNF-alpha, IL-6, CRP, MMP-2 and MMP-9 were remarkably decreased by TSG60, 120 mg x kg(-1) x d(-1) groups, which showed a dose-dependent effect.
CONCLUSIONTSG has the effect of anti-atherogenic and stabilizing plaque on the experimental rats with atherosclerosis, which are induced by the high cholesterol feeding and VitD3 injecting. The effect of TSG seems to be closely involved in regulating the expressions of MMP-2 and MMP-9, and inhibiting inflammation.
