Preparation of salbutamol polyclonal antibodies and development of indirect competitive enzyme-linked immunoassay.
- Author:
Shujie ZHANG
1
;
Yajing LEI
;
Xiaoqian XU
;
Weixing SHI
;
Shuqing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Albuterol; analysis; immunology; Animals; Antibodies; immunology; Enzyme-Linked Immunosorbent Assay; methods; Male; Rabbits
- From: Journal of Zhejiang University. Medical sciences 2013;42(1):19-24
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare the antibodies against salbutamol (SAL) with high sensitivity and to develop an indirect competitive enzyme-linked immunoassay (ic-ELISA) for fast detection of SAL.
METHODSThe New Zealand white rabbits were immunized with SAL in a small dose and long period mode. The method of ic-ELISA was optimized and adopted for the detection of a series of SAL samples, then the standard curve of SAL was established. The precision and the recoveries of the method were determined.
RESULTSThe antibodies with high sensitivity towards SAL were prepared with a IC50 of 12.21 ng/ml. The ic-ELISA method for SAL measurement was established, the recoveries of measurement was between 95%-105% and the CV was <3%.
CONCLUSIONThe antibodies against salbutamol have been prepared and an indirect competitive enzyme-linked immunoassay for fast and specific detection of SAL has been developed.
