Purification of arsenic-binding proteins in hamster plasma after oral administration of arsenite.
- Author:
Wenwen WANG
1
;
Min ZHANG
;
Chunhui LI
;
Yingjie QIN
;
Naranmandura HUA
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Arsenic; blood; Arsenites; administration & dosage; pharmacokinetics; Carrier Proteins; blood; Chromatography, High Pressure Liquid; methods; Cricetinae
- From: Journal of Zhejiang University. Medical sciences 2013;42(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo purify the arsenic-binding proteins (As-BP) in hamster plasma after a single oral administration of arsenite (iAs(III)).
METHODSArsenite was given to hamsters in a single dose. Three types of HPLC columns, size exclusion, gel filtration and anion exchange columns, combined with an inductively coupled argon plasma mass spectrometer (ICP MS) were used to purify the As-BP in hamster plasma. SDS-PAGE was used to confirm the arsenic-binding proteins at each purification step.
RESULTSThe three-step purification process successfully separated As-BP from other proteins (ie, arsenic unbound proteins) in hamster plasma. The molecular mass of purified As-BP in plasma was approximately 40-50 kD on SDS-PAGE.
CONCLUSIONThe three-step purification method is a simple and fast approach to purify the As-BP in plasma samples.
