Expression of 5-lipoxygenase in hippocampal CA1 neuronal damage following global cerebral ischemia in rats.
- Author:
Wenjian CHEN
1
;
Chengtan LI
;
Jianbo ZHAO
;
Xiaoyan ZHANG
;
Huayang HAN
;
Erqing WEI
;
Lihui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Arachidonate 5-Lipoxygenase; metabolism; physiology; Brain Ischemia; metabolism; pathology; CA1 Region, Hippocampal; metabolism; pathology; Disease Models, Animal; Hydroxyurea; analogs & derivatives; pharmacology; Lipoxygenase Inhibitors; pharmacology; Male; Neurons; drug effects; pathology; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2013;42(1):61-66
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine 5-lipoxygenase (5-LOX) expression and the effect of zileuton, a selective 5-LOX inhibitor,on hippocampal neuron injury induced by global cerebral ischemia in rats.
METHODSGlobal cerebral ischemia was induced by bilateral common carotid artery occlusion combined with hypotension in rats. 5-LOX expression was detected by Western blot analyses and 5-LOX localization was visualized by immunohistochemistry and double immunofluorescence methods. The 5-LOX inhibitor zileuton (10, 30, 50 mg/kg) was orally administered for 3 d after ischemia.
RESULTSThe 5-LOX expression was increased in the ischemic hippocampus on d1-7 (peaked at d3), and 5-LOX protein was primarily localized in neurons and translocated to the nuclei in the hippocampal CA1 region after ischemia. The 5-LOX inhibitor zileuton (30, 50 mg/kg) reduced ischemia-induced hippocampal neurons death 3d after ischemia.
CONCLUSION5-LOX is involved in global cerebral ischemic damage in rats, and the 5-LOX inhibitor zileuton has a protective effect on neuronal damage in the rat hippocampus following global cerebral ischemia.
