Effects of 17β-estrogen on expressions of C-reactive protein and its mRNA of vascular smooth muscle cells in rats.
- Author:
Li XU
1
;
Xinwu ZHANG
;
Xiaoming PANG
;
Lin CHEN
;
Kaige LIU
;
Yajun LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; C-Reactive Protein; genetics; metabolism; Cells, Cultured; Estrogens; pharmacology; Male; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; drug effects; metabolism; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2013;42(1):86-91
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of 17β-estrogen on expressions of C-reactive protein (CRP) and its mRNA in vascular smooth muscle cells(VSMCs).
METHODSImmunocytochemistry was used to detect CRP level in normal VSMCs. The expressions of C-reactive protein and p-ERK1/2 in Ang-II-stimulated VSMCs were evaluated with Western blot. C-reactive protein mRNA was examined with RT-PCR.
RESULTS17β-estrogen had no effect on cell morphology and C-reactive protein expression in normal VSMCs; however, C-reactive protein and mRNA, as well as p-ERK1/2 were decreased in Ang-II-stimulated VSMCs after 17β-estrogen treatment in a concentration-dependent manner.
CONCLUSION17β-estrogen may inhibit the expression of C-reactive protein and its mRNA in Ang-II-stimulated VSMCs via ERK1/2 signal transduction pathway in a concentration-dependent way.
