Determination of 7-ethyl-10-hydroxycamptothecin in microdialysates from rat brain with LC-MS/MS.
- Author:
Yang CAO
1
;
Qiaoyuan CHENG
;
Hongyang LU
;
Hengyi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Chemistry; Camptothecin; analogs & derivatives; analysis; Chromatography, Liquid; methods; Male; Microdialysis; Rats; Rats, Sprague-Dawley; Sensitivity and Specificity; Tandem Mass Spectrometry; methods
- From: Journal of Zhejiang University. Medical sciences 2013;42(1):98-102
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method for determination of 7-ethyl-10-hydroxycamptothecin (SN-38) in microdialysates from rat brain.
METHODSThe concentrations of SN-38 were measured by LC-MS/MS method with Agilent Eclipse Plus C18 (2.1 mm ×100 mm, 1.8 μm) reversed phase column using acetonitrile-0.1% methanoic acid as mobile phase with gradient elution at a flow rate of 0.3 ml/min and temperature at 35 degree. Multiple reaction monitoring using the precursor to product ion combinations of m/z 393.1→349.1 was performed to detect SN-38 in microdialysates from rat brain.
RESULTSBlank microdialysate had non-interference. The method was linear over the concentration range of 0.1015-1015 ng/ml (r=0.9995); and the lower limit of quantification (LOQ) was 0.1015 ng/ml. The recovery of assay for SN-38 ranged from 97.54%-100.60%. The intra- and inter-day precision and stability were both well. The concentrations of SN-38 in brain microdialysates presented pharmacokinetics process and achieved the peak after 220 min.
CONCLUSIONThe fully validated LC-MS/MS analytical method has high specificity and sensibility, which can be used effectively to analyze SN-38 in microdialysates from rat brain.
