Roles of protease-activated receptor-1 in thrombin-induced brain injury and neurogenesis in rats.
- Author:
Feifei LIU
1
;
Fuyi LIU
;
Lin WANG
;
Hua HU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Injuries; chemically induced; metabolism; pathology; Hippocampus; pathology; Male; Neurogenesis; drug effects; Rats; Rats, Sprague-Dawley; Receptor, PAR-1; agonists; physiology; Receptors, Thrombin; agonists; Thrombin; toxicity
- From: Journal of Zhejiang University. Medical sciences 2013;42(3):283-290
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the roles of protease-activated receptors (PARs) in thrombin-induced brain injury and neurogenesis in rats.
METHODSNinety male SD rats were randomly assigned to receive intra-hippocampus injection of NS, thrombin or specific agonists of 3 protease-activated receptors (PAR-1, PAR-3 and PAR-4), respectively. At 1,3 and 7 d after injection, the area of the hippocampus was determined with HE staining, the density and morphology of astrocyte were detected with GFAP staining, degenerated neurons were detected with Fluoro-Jade C staining, and the neurogenesis was examined with DCX staining.
RESULTSCompared to NS injection, the area of the hippocampus significantly increased at 1-3 d and decreased at 7 d after the injection of thrombin and PAR-1 agonist (P<0.05). In addition, injection of thrombin and PAR-1 agonist significantly increased the density of astrocyte and Fluoro-Jade C positive cells at 1-7 d after injection (P<0.05), and significantly increased the density of DCX positive cells at 3-7 d after injection(P<0.05). The injection of PAR-3 agonist and PAR-4 agonist had no affect on the area of the hippocampus, the density of astrocyte, Fluoro-Jade C positive cells and DCX positive cells.
CONCLUSIONThe activation of protease-activated receptor-1 may be related to the thrombin-induced brain injury and neurogenesis in rat hippocampus.
