Effects of shikonin on the proliferation and apoptosis of HL-60 cells.
- Author:
Zhi-Lu CHEN
1
;
Qi-Zhou DAI
;
Yong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Proliferation; drug effects; HL-60 Cells; Humans; Naphthoquinones; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2012;32(2):239-243
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the mechanism of shikonin for inducing the apoptosis of human promyelocytic leukemia cell HL-60.
METHODSThe effects of shikonin on the HL-60 cell proliferation were detected using MTT. The apoptosis rate was analyzed by Annexin-V/PI double staining. The expression level of the bcl-2 gene was detected using semi-quantitative reverse transcriptase PCR (RT-PCR), thus analyzing the correlation between the bcl-2 expression level and the apoptosis of HL-60.
RESULTSShikonin could inhibit the proliferation of HL-60 cells with the concentration range of 1-8 microg/mL in a time- and concentration-dependent manner. Two microg/mL shikonin could induce the apoptosis of HL-60 cells in a time-dependent manner. The expression level of bcl-2 was obviously down-regulated at 2 microg/mL shikonin.
CONCLUSIONSShikonin could induce the apoptosis of HL-60 cells. Its mechanism was correlated with down-regulation of the expression level of bcl-2.
