Effects of Bushen Shuji Granule on IL-6 in the culture fluid sample of fibroblast cells from the synovial liquid of the ankylosing spondylitis patients.
- Author:
Jun-Ling ZHU
1
;
Xiao-Ping YAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Drugs, Chinese Herbal; pharmacology; Female; Fibroblasts; drug effects; secretion; Humans; Interleukin-6; metabolism; Male; RNA, Messenger; genetics; Rats; Rats, Sprague-Dawley; Spondylitis, Ankylosing; Synovial Fluid; metabolism; Synovial Membrane
- From: Chinese Journal of Integrated Traditional and Western Medicine 2012;32(2):257-260
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of Bushen Shuji Granule (BSG) on inhibiting the interleukin 6 (IL-6) level in the synovial fluid sample of fibroblast cells from the ankylosing spondylitis (AS) patients.
METHODSUsing serum pharmacologic method, the IL-6 level in the culture fluid sample of fibroblast cells was observed by ELISA method with different concentrations of medicated serum containing BSG. The IL-6 level at the mRNA level was detected using reverse transcriptase-polymerase chain reaction (RT-PCR). The vehicle serum and sulfasalazine (SSZ) serum were taken as controls.
RESULTSResults of ELISA showed the IL-6 level in the AS group was more than that in the vehicle serum group with obvious statistical difference. BSG could obviously inhibited the IL-6 level, showing statistical difference when compared with the vehicle serum group. Besides, obvious dose-dependent correlation existed between BSG and its inhibition on fibroblast proliferation. And the IL-6 level at the mRNA level in the AS group was higher than that in the vehicle serum group, showing statistical difference by semi-quantitative analysis.
CONCLUSIONBSG could play its clinical role of anti-inflammation and anti-fibrosis through inhibiting the IL-6 level in the culture fluid sample of fibroblast cells.
