Experimental study on the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats.
- Author:
Ling LU
1
;
Lian-Qun JIA
;
Wei MA
Author Information
- Publication Type:Journal Article
- MeSH: Albumins; metabolism; Animals; Drugs, Chinese Herbal; pharmacology; Female; Heat-Shock Proteins; metabolism; Ileum; drug effects; metabolism; Male; Medicine, Chinese Traditional; Proteome; analysis; Proteomics; Qi; Rats; Rats, Sprague-Dawley; Syndrome; Trypsin; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2012;32(5):685-688
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify the ileum tissue proteins differentially expressed in Pi-qi deficiency syndrome rats using proteomic approach.
METHODSThirty-seven rats were randomly divided into 3 groups, i. e., the normal control group (Group 1), the Pi-qi deficiency syndrome model group (Group 2), and the reserpine group (Group 3). The Pi-qi deficiency syndrome model was established using excessive exerting combined with irregular diet, and peritoneally injecting Reserpine Injection (1 mg/mL) respectively. The ileum tissues were separated after identified fuzzy method. The differentially expressed proteins were separated with two dimensional electrophoresis (2-DE), analyzed by Mass Spectrometry, and identified by MASCOT Software.
RESULTSThree proteins were differentially expressed in Group 2 and nine proteins were differentially expressed in Group 3 (P < 0.05).
CONCLUSIONPi-qi deficiency syndrome was closely related with decreased expression of albumin as well as increased expressions of trypsin and glucose-regulated protein 78.
