Expression and amplification of steroid receptor coactivator-3 gene in colorectal carcinoma and its clinicopathological significance.
- Author:
Sui-de ZENG
1
;
Dan XIE
;
Feng LIN
;
Chang-xi WANG
;
Yu TAO
;
Meng ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; genetics; Colorectal Neoplasms; genetics; pathology; Female; Gene Expression; Histone Acetyltransferases; genetics; Humans; Male; Middle Aged; Neoplasm Staging; Nuclear Receptor Coactivator 3; Trans-Activators; genetics
- From: Chinese Journal of Gastrointestinal Surgery 2005;8(1):67-70
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression and amplification of steroid receptor coactivator- 3(SRC- 3) gene in colorectal carcinoma (CRC) and its clinicopathological significance.
METHODSImmunohistochemistry and fluorescence in situ hybridization (FISH) were used to detect the expression and amplification of SRC- 3 gene in CRC, and its association with patient's clinical pathological features was analyzed.
RESULTSA total of 60 patients with CRC were studied. SAR- 3 proteins were overexpressed in 23 cases (38% ). There was a significant association between SAR- 3 overexpression and neoplasm staging (P< 0.01). SRC- 3 protein was overexpressed in 62% of patients with Dukes C or D stage, whereas SRC- 3 protein was normally expressed in 74% of patients with Dukes A or B stage. As for FISH study, 47 cases were informative. High- level amplification of SRC- 3 gene was detected in 6 cases(13% ) and all showed overexpression of SRC- 3 protein. Low- level amplification of SRC- 3 was observed in 9 cases (19% ). Overexpression of SRC- 3 was detected in 6 cases. The remaining 9 of 32 patients(28% ) without amplification of SRC- 3 gene were observed with overexpression of SRC- 3 protein. In addition, 91% patients with CRC were found overexpression of SRC- 3 as well as overexpression of P53.
CONCLUSIONThe abnormal expression of SRC- 3 gene might impact on the function of P53 and development of CRC. There might exist some unknown mechanisms other than gene amplification of SRC- 3 to regulate its encoded protein expression in CRC.