- Author:
Baoxing FAN
1
;
Jingfen SUN
;
Kaitai ZHANG
;
Dechang WU
Author Information
- Publication Type:Journal Article
- From: Chinese Journal of Lung Cancer 2002;5(5):321-325
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo profile the expression patterns of 60 lung cancer related genes in human bronchial epithelial cell (BEP2D) and alpha-particle induced malignantly transformed cell (R15Hp35T-2).
METHODSSixty lung cancer related cDNAs were micro-arrayed onto the microscope slides using Cartesian PixSys5500 cDNA Microarray machine. Total RNA from BEP2D cell and R15Hp35T-2 cell was extracted and labeled by fluorescent dye. The labeled probe was then hybridized with the cDNA.
RESULTSCompared with the BEP2D cell, 27 genes up-regulated and 7 down-regulated in the R15Hp35T-2 cell. The expression abundance of most tumor suppressor genes were similar in the two kinds of cells, however, most oncogenes and growth factor genes were overexpressed in R15Hp35T-2 cell.
CONCLUSIONSIn malignantly transformed human bronchial epithelial cell model induced by alpha-particle, some oncogenes and growth factor genes may promote the malignant transformation together.