- Author:
Jianjun QIN
1
;
Qinghua ZHOU
;
Yang QIN
;
Zhilin SUN
;
Feng ZHAO
;
Zefang SUN
;
Yanping WANG
;
Cheng YI
;
Wen ZHU
Author Information
- Publication Type:Journal Article
- From: Chinese Journal of Lung Cancer 2003;6(3):169-171
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo construct recombinant adenoviral vector carrying Smad3D or Smad7 by a simplified means.
METHODSBased on AdEasy System, adenoviral backbone plasmid vector and shuttle vector carrying the gene of interest were transferred into E.coli BJ5183 by chemical transformation methods in special order. The homologous recombination was performed.
RESULTSRecombinant adenoviral vector pAd-Smad3D and pAd-Smad7 were constructed successfully, which were confirmed by restriction enzyme digesting.
CONCLUSIONSRecombinant adenoviral vector may be constructed quickly and efficiently in E.coli by sequential chemical transformation methods.