Study on the growth-inhibiting effect of anticancer ketonon on lung cancer cell line and its mechanism.

Author: Jianjun QIN ¹ ; Qinghua ZHOU ; Shulan YUAN ; Yanping WANG ; Xiaohe CHEN ; Jintao HE
Author Information

1. Laboratory of Lung Cancer Molecular Biology, Key Laboratory of Sichuan Province, and Department of Thoracic and Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R.China.
Publication Type:Journal Article
From: Chinese Journal of Lung Cancer 2003;6(4):261-263
CountryChina
Language:Chinese
Abstract:
BACKGROUNDTo observe the growth-inhibiting effect of anticancer ketonon on A549 cell line and PLA-801D cell line and to explore its mechanism based on the antineoplastic effects of Tanshinon.
METHODSA549 and PLA-801D cell lines were treated with anticancer ketonon by techniques of cell culture in vitro . The growth curves and dose-effect curves were drawn up. The ability of clone formation was determined. It was observed and analysed by light microscopy and flow cytometry.
RESULTSThe growth of A549 and PLA 801D cell lines was evidently inhibited. Ability of clone formation was inhibited. The apoptosis index of cells was increased after treated with anticancer ketonon and the cell cycle was blocked at G0/G1 phase.
CONCLUSIONSAnticancer ketonon can significantly inhibit the growth of human lung cancer cells probably through inducing the apoptosis of cancer cells.