Studies on HPLC fingerprint of co-hirudo injection.
- Author:
Xiao-huan YUAN
1
;
Xu-dong YANG
;
Chun-tao WANG
;
Guo-mei ZU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; chemistry; Hypoxanthine; chemistry; Leeches; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2008;33(15):1825-1827
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEUsing HPLC To determine hypoxanthine in co-hirudo injection for establishing its HPLC fingerprint, and evaluating its internal quality.
METHODThe chromatographic separation was performed on a Kromasil C18 column (4.6 mm x 250 mm,5 microm). A linear gradient elution with A (0.01 mol x L(-1) x KH2PO4) and B (50% methanol) was used, the flow rate was 0.8 mL x min(-1), the detection wavelength was set at 254 nm, and the column temperature was at normal.
RESULTHypoxanthine was used as the reference substance in the fingerprint of co-hirudo injection, it showed 15 common peaks and theirs similarity threshod was 0.97.
CONCLUSIONThis method was accurate, repeatable and useful for the quality control of co-hirudo injection.