Studies on chemical constituents and antibacterial activity from n-butanol extract of Sarcandra glabra.
- Author:
Ke YUAN
1
;
Jian-xin ZHU
;
Jin-ping SI
;
Hai-ke CAI
;
Xiao-dan DING
;
Yuan-jiang PAN
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; chemistry; isolation & purification; pharmacology; Butanols; chemistry; Drugs, Chinese Herbal; chemistry; isolation & purification; pharmacology; Magnetic Resonance Spectroscopy; Magnoliopsida; chemistry; Staphylococcus aureus; drug effects
- From: China Journal of Chinese Materia Medica 2008;33(15):1843-1846
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the chemical constituents and the antibacterial activity from n-butanol extract of Sarcandra glabra.
METHODThe compounds were isolated by Diaion HP-20, Sephadex LH-20, MCI CHP-20 and silica gel column chromatographic methods. Their structures were identified on the basis of physicochemical and spectroscopic analysis. The antibacterial effect of the compounds were measured against Staphylococcus aureus by filterpaper slice method, finally the antibacterial ring in each group was recorded after 24 hours.
RESULTSeven constituents were isolated and elucidated as 5, 7, 3', 4'-tetrahydroxy-6-C-beta-D-glucopyranosylflavanone (1), kaempferol-3-O-beta-D-glucuronide (2), fraxidin (3), isofraxidin (4), isofraxidin-7-O-beta-D-glucopyranoside (5), kaempferol (6), pinostrobin (7). Diameters (in mm) of antibacterial ring in the compounds 2, 5, 6 were orderly recorded as follows: 14.67 +/- 0.08, 11.14 +/- 1.06, 8.26 +/- 1.26 and the compound 4 is not effective.
CONCLUSIONCompounds 1-3 and 5 were isolated from S. glabra for the first time.
