Rapid determination of thiabendazole residue in barbary wolfberry fruit by ion exchange chromatography and fluorescence detection.
- Author:
Jie ZHAO
1
;
Qiang HE
;
Xiang-hong KONG
;
Jian-hua LI
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Ion Exchange; methods; Fruit; chemistry; Lycium; chemistry; Reproducibility of Results; Spectrometry, Fluorescence; Thiabendazole; analysis; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(15):1853-1855
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo developed a method for the determination of thiabendazole residues in barbary wolfberry fruit by ion exchange chromatography (IEC) with fluorescence detection.
METHODThe samples were extracted with 0.1% H3PO4. Chromatographic analysis was performed on a Supelcosil LC-SCX (4.6 mm x 250 mm, 5 microm) column eluted with 0.1 mol x L(-1) KH2PO4 (pH 3.0)-acetonitrile (70:30) and detection at lamdaex = 307 nm and lamdaem = 359 nm.
RESULTThiabendazole in measured samples was separated completely. The calibration curve was linear at the range of 0.0005-0.02 mg x L(-1) with good precision and accuracy.
CONCLUSIONThe proposed method was satisfactorily applied to the analysis of thiabendazole residues in barbary wolfberry fruit.
