Studies on absorption kinetics of scopoletin in rat stomachs and intestines.
- Author:
Yu-feng XIA
1
;
Yue DAI
;
Qing-yu MENG
;
Qiang WANG
;
Ling-ling QIU
Author Information
- Publication Type:Journal Article
- MeSH: Absorption; Animals; Chromatography, High Pressure Liquid; Female; Hydrogen-Ion Concentration; Intestinal Absorption; Intestines; metabolism; Male; Rats; Rats, Sprague-Dawley; Scopoletin; pharmacokinetics; Spectrophotometry, Ultraviolet; Stomach; metabolism
- From: China Journal of Chinese Materia Medica 2008;33(15):1890-1894
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study absorption kinetics of scopoletin in rat stomachs and intestines.
METHODRats was cannulated for in situ recirculation. UV and HPLC methods were used to determine the concentrations of phenolsulfonphthalein and scopoletin, respectively.
RESULTThe absorption rates in rat stomachs at 2 h after administration was 76.31%; The absorption rates at colon, duodenum, ileum and jejunum were 46.25%, 40.54%, 38.21%, 32.77%, respectively. The absorption rate constant (Ka) at concentrations of 10.0144, 20.0288-40.0576 mg x L(-1) in intestine were 0.6434, 0.6137, 0.5970 h(-1), respectively. The Ka of scopoletin at pH of 6.0, 6.8 and 7.4 in intestine were 0.6217, 0.6033, 0.6137 h(-1), respectively.
CONCLUSIONThe concentrations and pH values of scopoletin solution had no distinctive effect on the absorption kinetics. The absorption of scopoletin was a first-order process with passive diffusion mechanism. Scopoletin was well absorbed at stomachs and intestines in rats. Colon was the best absorption site of scopoletin, which suggest that a sustained-release preparation should be suitable for this compound.
