Extraction of triterpenoids from fruiting corrected bodies of Ganoderma lucidum by supercritical fluid extraction.
- Author:
Shi-hua SONG
1
;
Xiao-bin JIA
;
Yan CHEN
;
Bin CHEN
;
Li-jing WANG
;
Cheng-lei ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Supercritical Fluid; methods; Fruiting Bodies, Fungal; chemistry; Reishi; chemistry; Spectrophotometry, Ultraviolet; Triterpenes; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(17):2104-2107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the extracting condition for triterpenoids from the fruits of Ganoderma lucidum by supercritical fluid extraction (SFE).
METHODOptimum extraction conditions were studied by orthogonal tests. The total triterpenoids were determined by ultraviolet spectrophotometry and ganoderic acid B was determined by RP-HPLC.
RESULTThe optimal extraction conditions were that the pressure 25 MPa, the temperature was 45 degrees C, the extraction time was 1.5 hour, and the ethanol was employed as modifier carrier at the volume of 3 mL x g(-1).
CONCLUSIONSFE is superior and it is feasible to extract triterpenoids from fruits of Ganoderma lucidum.
