Study on qualitative and quantitatire methods for Ceratostigma willmottianum.
- Author:
Ai-min WANG
1
;
Yong-lin WANG
;
Yong-jun LI
;
Hong SU
;
Ping ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Chromatography, Thin Layer; methods; Drugs, Chinese Herbal; chemistry; Naphthoquinones; analysis; Plumbaginaceae; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(17):2130-2132
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the qualitative and quantitative methods of Caratostigma willmottianum.
METHODThe roots of Radix Ceratostigmae were identified by TLC with the authentic medicinal material and plumbagin as the reference. The contents of plumbagin in Radix Ceratostigmae were determined by HPLC.
RESULTThe TLC method was simple and specific. A Diamonsil C18 column was used. The mobile phase was methanol-water (75:25). Plumbagin in the sawple extract was separated well. The linear range of plumbagin was 33.6-313.6 ng. The average recovery of plumbagin was 100.3% and RSD was 1.9%.
CONCLUSIONThe methods can be used for identification and determination of plumbagin in C. willmottianum.
