Determination of lobetyolin in root of Codonopsis tangshen from various cultivation areas by high-performance liquid chromatography.
- Author:
Dan SONG
1
;
Xue-mei CHENG
;
Long-yun LI
;
Guo-yue ZHONG
;
Zheng-tao WANG
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; methods; Codonopsis; chemistry; Drugs, Chinese Herbal; chemistry; Plant Roots; chemistry; Polyacetylenes; analysis; Quality Control
- From: China Journal of Chinese Materia Medica 2008;33(17):2133-2135
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas.
METHODA Supelco Discovery C18 Column (4.6 mm x 250 mm, 5 microm) was used with acetonitrile-0.5% acetic acid in water (20:80) as the mobile phase and UV detection was at 268 nm.
RESULTTwenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0.0403-0.9667 mg x g(-1).
CONCLUSIONThe method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.
