Optimization of enzymatic extraction of effective constituents from fibrous roots of Salvia miltiorrhiza by central composite design and response surface method.
- Author:
Gang LU
1
;
Guo-dong DU
;
Jun-ru WANG
;
Zong-suo LIANG
Author Information
1. The College of Science, Northwest A & F University, Yangling 712100, China.
- Publication Type:Journal Article
- MeSH:
Diterpenes, Abietane;
Drugs, Chinese Herbal;
chemistry;
Phenanthrenes;
chemistry;
Plant Roots;
chemistry;
Salvia miltiorrhiza;
chemistry
- From:
China Journal of Chinese Materia Medica
2008;33(16):1976-1981
- CountryChina
- Language:Chinese
-
Abstract:
The fibrous roots are the residues of production of cut crude drug of Danshen (Salvia miltiorrhiza). Enzymatic pretreatment and ultrasonic extraction are beneficial to extract effective constituents from fibrous roots more effectively. The present research was to optimize the enzymatic parameters by the central composite design and response surface method. Under the best conditions, the yields of total tanshinones and total salvianolic acids in the extracts of enzymatic pretreatment increased by 113.92% and 30.64%, comparing with the non-enzymatic extraction, respectively. TLC analysis also showed that the types of effective constituents in the two samples were not affected by enzymatic hydrolysis. Meanwhile, the complex correlation coefficients of the mathematical models were high, which provided a good prediction.
