Anti-tumor mechanism of active components from extract of Actinidia rufa root.
- Author:
Guo-biao LIN
1
;
Zhen-guo ZHONG
;
Wen-yan ZHANG
;
Feng-fen ZHANG
;
Xi-hui CHEN
;
Chu-sheng HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Actinidia; chemistry; Apoptosis; drug effects; Cell Line, Tumor; Drugs, Chinese Herbal; chemistry; pharmacology; Flow Cytometry; Humans; Immunohistochemistry; Plant Roots; chemistry
- From: China Journal of Chinese Materia Medica 2008;33(16):2011-2014
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe effect and mechanism of n-Butanol lysate of alcohol extracts from Actinidia rufa root (monomer of R6,R8).
METHODTunel, Wright's stain with Giemsa's stain dyeing, and Hoechst 33258-PI double dyeing assay were used to detect the apoptosis of SGC7901 tumor cells treated with R6, R8. The SGC7901 tumor cells were randomly divided into control group and two treatment groups administered 0.05 g x L(-1) R6, R8, respectively, for 72 h). FCM assay was used to detect the apoptosis. Agarose electrophoresis assay was used to detect DNA strand break of tumor cells and reveal anti-tumor action mechanism.
RESULTThe apoptosis percentage of the tumor cell in 24 h, 48 h, 72 h was (17.08 +/- 2.78)% , (29.68 +/- 2.96)%, (52.46 +/- 3.81)%; (14.75 +/- 2.14)%, (27.35 +/- 3.79)%, (45.64 +/- 5.24)%, respectively, for the treatment group, significantly higher than that in the control group (1.94 +/- 1.55)%, (2.78 +/- 1.84)%, (11.8 +/- 2.79)% (P < 0.01) by tunnel assay. Wright's stain with Giemsa's stain dyeing assay, Hoechst 33258-PI and FCM double dyeing assay showed same action. R6 and R8 had the effect of inducing the DNA histogram of tumor cells (P < 0.01).
CONCLUSIONThe anti-tumor mechanisms may be associated with inducing the injury of DNA and stimulating apoptosis.
